Distribution and drug resistance of pathogenic bacteria in blood culture

OBJECTIVE To investigate the distribute of pathogenic bacteria in blood culture,and observe the drug resistance of common pathogenic bacteria in our hospital.METHODS Blood samples were cultivated with Bact/Alert3D automated hemanalysis system,pathogenic bacteria were identified with VITEK 2 compact,drug sensitivity test was carried out by using K-B method and the test results were analyzed with WHONET 5.4 software.RESULTS Among the 1578 clinical blood samples,257 strains of pathogenic bacteria were isolated,the positive rate was 16.3%,Gram-positive bacteria were the most common pathogens(41.6%),then Gram-negative bacteria(40.1%) and fungi(18.3%).The coagulase negative staphylococcus were the most common in all bacteria(24.5%),then E.coli(24.1%) and Candida albicans(10.9%).The pathogenic bacteria were isolated from ICU sickroom(40.5%) then Blood internal medicine sickroom(8.6%) and Rheumatism immunity sickroom(7.8%).The Gram-positive bacteria were sensitve to Teicoplanin,Vanconmycin and linezolid.The Gram-negative bacteria were sensitive to imipenem,meropenem and piperacillin/tazobactam;the sensitivity of C.albicans to Fluconazole,the sensitivity of Candida glabrate and Candida tropicalis to Itraconazole.CONCLUSION The microorganisms isolated from blood culture are widely distributed and the drug resistance to common antibiotics is high,the early detection of the isolated pathogens and the drug resistance analysis are very important in guiding the clinical administration.It suggests that antibiotic should be used rationally so as to reduce the drug resistance of bacteria.