Determination of Three Major Flavonoids in Citrus paradisi cv.Changshan Huyou Fruit

Objective: To establish a method for determining naringin,hesperidin,and neohesperindin in Citrus paradisi cv.Changshan Huyou fruit and compare their contents in different parts such as peel,pulp and seeds.Methods: The contents of naringin,hesperidin and neohesperindin were determined by a reversed-phase HPLC method.Methanol extracts of samples and the standard substances were separated on a YMC C18 column(4.6 mm × 250 mm,5μm) using a mobile phase made up of acetonitrile and water(23:77,V/V) at a flow rate of 1 mL/min and detected by a UV detector at a wavelength of 283 nm.The column temperature was set at 25 ℃.Results: The calibration curves were linear in the range of 3.22-1030μg/mL for naringin,0.59-380μg/mL for hesperidin,and 0.077-990μg/m for neohesperindin,respectively.The average recoveries of naringin,hesperidin and neohesperindin were 98.43%(RSD = 4.07%),98.70%(RSD = 4.30%) and 99.90%(RSD = 3.47%),respectively.The contents of naringin,hesperidin,neohesperindin were the highest in the peel and the lowest in the seeds of Citrus paradisi cv.Changshan Huyou fruit.Conclusions: This method is simple,accurate,reliable and reproducible and can therefore be used for the quality control of Citrus paradisi cv.Changshan Huyou fruit.