RP-HPLC DETERMINATION OF NARINGIN AND HESPENIDIN IN POMELO PEEL

Naringin and hesperidin in pomelo peel were separated on a μ-Bondapak C18 column (250 mm×4. 6 mm, 5 μm), using a mixed solution composing methanol and diluted acetic acid (1 + 199) in the ratio of 38 to 62 by volume, as the mobile phase. The sample of pomelo peel was sliced, dried and powdered to have a grainity of 850 -600 μm. The powdered sample was then defatted by refluxing with petroleum ether at 80℃ for 2. 5 h and the components to be determined in the sample were then extracted by refluxing with methanol at 80℃ for 3 h. A diluted methanol extract was then introduced into the separation column at a column temperature of 25℃ with a flow-rate of 0. 8 mL·min-1. Linear calibration curves were obtained in the ranges of 20-60 mg·L-1 for naringin and of 10-30 mg·L-1 for hesperidin, and the average recoveries found were 99. 3% and 97. 8% respectively. The RSD s obtained were less than 3. 2% for naringin and less than 3. 7% for hesperidin.