2007•Anhui nongye kexueRequires access

Determination of Naringin in Leaves of Citrus grandis(L.)Osbeck by the Method of HPLC

Lan Song

Abstract

To establish a method for the determination of naringin in leaf of Citrus grandis(L.)Osbeck by HPLC,the chromatographic condions were as follows:Kromasil C18 column with methel-water-acetic acid(35∶61∶4) as mobile phase,the detection wavelength at 283 nm and the flow rate being 1.0 ml/min.The results indicated a linearity of naringin in the range of the average recovery was 101.27%(RSD=1.41).The conclusion was the method was rapid and precise,and could be used to determine naringin in leaves of Citrus grandis(L.)Osbeck.

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Key concepts: Naringin, Chromatography, Chemistry, High-performance liquid chromatography, Acetic acid, Biochemistry