Studies on pharmacokinetics and residues of Chloramphenicol in perch( Lateolabras janopicus) in vitro

The pharmacokinetic and residual characteristics of Chloramphenicol in healthy perch were studied by using the High Performance Liquid Chromatography.Data were analyzed with the pharmacokinetic computer program MCP KP.The results showed that the plasma concentration time course of Chloramphenicol can be described by a one compartment open model with the first order absorption after oral administration(80 mg/kg).Its theoretical equation was as follows: C blood =30.815( e -0.061 4t -e -0.662t ); and the main pharmacokinetic parameters were those: K a was 0.662 h, T 1/2K a was 1.047 h; C max was 21.093 μg/ml, T max was 3.961h,AUC was 438.31 mg/L·h,Kel was 0.061 4h, T 1/2k was 11.293h.The kinetics process of Chloramphnicol in tissues can all be described by the biexponential equation with the first order absorption.The elimination characteristics of multiple dose administration(40 mg/kg)can be described by the equations as follows: C blood =3 40 e -0.403t ?C muscle =4.342e -0.458t ?C liver =2.152 e -0.239t ?C kidney =64.155 e -1.443t .According to the equations ,the time taken by the concentrations of Chloramphnicol dropping to 0.01 μg/ml in blood,muscle,liver,and kidney was 14.164,13.261,22.476,6.075 days respectively.