Radioimmunoimaging and biodistribution of ~(131)I labeled anti-CEA monoclonal Antibody C50 in nude mice bearing human breast carcinoma xenografts

Objective To study the Radioimmunoimaging and distribution of ~(131)I labeled anti-CEA monoclonal antibody(McAb) C50 and normal mice IgG in made mice bearing human breast Carcinoma and to provide the basis for clinical use. Methods 3.7 MBq/0.1 ml labeled antibody was injected into the tail vein in each respectively SPECT were measured after 24th 48th 96th 120th hours interval of injection; Radioactivity ratio of tumor end non-tumor(T/NT), Percent injected dosage of every gram tissue(%ID/g) were measured after 48th, 96th, 120th hours interval to death. Results Positive of SPECT were found during 24th to 120th hour after the ~(131)I-C50 injection, Positive rates was 50%. The peak value of T/NT Tumor blood was 3.07±1.01 at 120th hour. But non-concentration of ()~(131)I-mIgG were found during 24th to 120th hour, which was distributed evenly in body. Conclusion C50 had affinity to breast carcinoma which over expressed CEA in nude mice bearing human breast Carcinoma. C50 is prospective to be used as the direct Carrier of breast carcinoma diagnosis.