Radioimmunoimaging and biodistribution of ~(131)I labeled anti-TPS monoclonal Antibody in nude mice bearing human breast Carcinoma

Objective To study the Radioimmunoimaging and distribution of 131 I labeled anti TPS monoclonal antibody and normal mice IgG in made mice bearing human breast Carcinoma and to provide the basis for clinical use. Methods 3 7 MBq/0 1 ml labeled antibody was injected into the tail vein in each respectively SPECT were measured after 24th 48th 96th 120th hours interval of injection; Radioactivity ratio of tumor end non tumor(T/NT), Percent injected dosage of every gram tissue(%ID/g) were measured after 48 96 120 hours interval to death. Results Positive of SPECT was found during 24th to 120th hour after the 131 I TPS injection, Positive rates was 58%. The peak value of T/NT Tumor blood was 2 51±0 71 at 120th hour. But non concentration of 131 I mIgG was found during 24th to 120th hour, which was distributed evenly in body. Conclusion Anti TPS monoclonal Antibody had affinity to breast carcinoma which over expressed TPS in nude mice bearing human breast Carcinoma. TPS monoclonal Antibody is prospective to be used as the direct Carrier of breast carcinoma diagnosis.