Establishment of regeneration system of maize embryo leaf of inbred lines in northeast

3 mm long leaf segments of Zea mays L. from 7-day-old seedlings were c ultured on inducing nutrient medium to study on establishment of regeneration sy stem of maize embryo leaf of inbred lines in northeast. The results showed that the ability of callus induced maize embryo leaf was obviously different from pos itions, genotype and plant hormones at different concentrations. Inducing rate o f embryogenic callus gradually reduced from bottom to top of embryo leaf. 2, 4-D was the most factor to callus induction. In addiction, the medium supplemented with certain AgNO3 was profitable to induce callus tissues. Now we discussed fac tors of affecting differentiation of shoots and established regeneration system of maize embryo leaf of inbred lines in northeast.