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Determination of Quercetin in Yiyi by HPLC

Peng Xia

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Abstract

Objective To determination the contents of quercetin in Yiyi. Method HPLC method was used. Chromatographic condition included inertsil ODS-3 column (4.6 mm×200 mm, 5 μm) and the mobile phase consisting of methanol-0.4% phosphoric acid, gradient elution with a detection wavelength of 360 nm. Results The calibration curves were linear in the range of 0.586～11.72 μg for quercetin, r=0.9999, average recoveries of quercetin were 99.52%, RSD was 1.2% (n=3). Conclusion This method was simple and quickly, accurat and easy to operate.

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What this paper is about

Objective To determination the contents of quercetin in Yiyi. Method HPLC method was used. Chromatographic condition included inertsil ODS-3 column (4.6 mm×200 mm, 5 μm) and the mobile phase consisting of methanol-0.4% phosphoric acid, gradient elution with a detection wavelength of 360 nm. Results The calibration curves were linear in the range of 0.586～11.72 μg for quercetin, r=0.9999, average recoveries of quercetin were 99.52%, RSD was 1.2% (n=3). Conclusion This method was simple and quickly, accurat and easy to operate.

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Available abstract

Objective To determination the contents of quercetin in Yiyi. Method HPLC method was used. Chromatographic condition included inertsil ODS-3 column (4.6 mm×200 mm, 5 μm) and the mobile phase consisting of methanol-0.4% phosphoric acid, gradient elution with a detection wavelength of 360 nm. Results The calibration curves were linear in the range of 0.586～11.72 μg for quercetin, r=0.9999, average recoveries of quercetin were 99.52%, RSD was 1.2% (n=3). Conclusion This method was simple and quickly, accurat and easy to operate.

Key concepts: Chromatography, Quercetin, Phosphoric acid, Gradient elution, Calibration curve, Chemistry, High-performance liquid chromatography, Detection limit

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