Study on integrase I gene in imipenem-resistant Pseudomonas aeruginosa.

Aim To investigate the source and drug-resistant mechamism of integrase I gene(including intI1 and qacE △1-sulI)in imipenem-resistant Pseudomonas aeruginosa(IRPA)isolated from the clinical specimens in our hospita.Methods Bacteria were identified and susceptibility test were conducted by VITEK-AMS 60 auto microbial system.The integron genetic markers were analyzed from 60 isolated strains of P.aeruginosa by polymerase chain reaction(PCR)and verificated by DNA sequencing.Results In 30 strains of IRPA,the positive rates of gene of intI1,qacE△1-sulI were 23(76.7%),11(36.7%);in other 30 strains of imipenem-sensitivity,the positive rates of gene of intI1,qacE△1-sulI were 12(40.0%),3(10.0%).The positive genes were main from neurosurgery department,second were nerve medicine department and surgical ICU ward.Conclusion Integrase I gene(including intI1 and qacE△1-sulI)is associated with multi-drug resistance of P.aeruginosa,especially intI1 genes were very high,the higher level of intI1 genes is possibly an relevant to drug resisstance and multi-drug resistance of P.aeruginosa.In order to reduce the drug-resistance strains,antibiotics should be used reasonably in addition to strengthening the monitoring of drug-resistant genes of pathogens.