2010•Zhongguo yufang shouyi xuebaoRequires access
Cloning and analysis of glycoprotein gene of rabies virus isolated from Guangdong province
Jiang Biao, Zhen JiaLin, Liang HongRu, Hui Xu, Xiaofeng Guo
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Abstract
The glycoprotein (G) gene of rabies virus (GD33), isolated from salivary of a health dog in Guangdong province, was amplified by RT-PCR, cloned and sequenced. Comparison results showed that the nucleotides and deduced amino acids sequence of G genes of GD33 and HEP-Flury share 97.1 % and 99.2 % homology, respectively, especially in transmembrane and intramembrane regions. In addition, an Arg to Gln mutation was found in G gene of GD33, HEP-Flury and FluryLEP strains at the 333 amino acid site. These results indicated that the GD33 isolate is low virulent strain.