2012•Journal of the South China Agricultural UniversityRequires access
Analysis of N and G Gene of Rabies Virus Isolated from Pig
Ying Zhang, Xiangyin Liu, Yongwen Luo, Yang XianFeng, Huang YongLiang, He-He Shi, Qiuling Fu, Yang Youtian, Xiaowei Wu, Xiaofeng Guo
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Abstract
To understand the difference of the nucleoprotein(N) gene and glycoprotein(G) gene between the strain of pig origin and other rabies viruses.The N and G genes of rabies virus isolated from the brain of a rabid pig in Guangdong Province were amplified by RT-PCR,cloned,sequenced and compared with the genes of other rabies virus.The results showed that the sequence of nucleotides and amino acids of N genes of GD-SH01 shared 84.3%-98.0% and 92.5%-99.3% similarity,and G genes of GD-SH01 shared 80.4%-98.2% and 87.8%-99.6% similarity,respectively,compared to other viruses.