Distribution of human papillomavirus genotype and its correlation to HPV cervical infection

Objective:To investigate the distribution character of human papillomavirus(HPV) infection in the female and explore the risk of cervical diseases associated with HPV genotype.Methods:A total of 908 cervical cell samples were used to detect HPV genotypes with HybriMax.Among them 131 women who were gynecological inpatients were choosed,they were classified into 5 groups according to their pathologic examination: chronic cervicitis group,CINⅠgroup,CINⅡ group,CINⅢ group and cervical cancer group.The distribution data of HPV genotype was statistically analyzed in Zhejiang Province.Results:Eighteen genotypes were found by HybriMax in 21 HPV genotypes,except HPV35,HPV43 and HPV59.The HPV infection rate among the 908 cases is 30.3%(275∕908),among which,the infection rate of ≤ 35-year-old female is 40.2%(119/296),as the age increases,the infection rate decreases,but HPV infection rate increases in women over the age of 55.The more serious the cervical diseases were,the higher positive rates of HPV infection would be.The infection rates of HPV at histological phase of chronic cervicitis,CINⅠ,CINⅡ,CINⅢ and cervical cancer group were 40.7%(22/54),49.8%(11/23),64.0%(16/25),78.9%(15/19),90.0%(9/10),respectively,all higher than that of the normal group 13.1%(21/160)(P0.05).The distribution of the HPV genotype is differently distributed in various histologic-grade diseases,HPV52,58,16,18,39 were common in normal group,and HPV16,52,58,18,39 were common in abnormal group.The most common genotype was HPV16(70.0%) in cervical cancer group.Conclusion:HPV infection is closely related to cervical diseases.HPV16 infection is the most common genotype in cervical cancer.HPV-DNA genotyping is of great importance for high-risk group screening of cervical cancer,clinical diagnosis and prognosis.