Preliminary analysis of human Papillomavirus genotype distribution in cervical diseases

Objective To investigate the distribution of Human Papillomavirus(HPV)genotype in cervical diseases and explore the risk of cervical cancer associated with HPV genotype.Methods 318 patients infected with high-risk HPV(HC-Ⅱ)were classified into 4 groups according to their pathologic examination:cancer group,HSIL group,LSIL group,and chronic cervicitis group.HybriMax were used to detect HPV genotype in cervical tissues previously detected by HC-Ⅱ from these patients.The distribution data of HPV genotype were statistically analysed.Results Nineteen genotypes were found by HybriMax in total 21 HPV genotype,except HPV44 and HPV56.The HPV genotype is differently distributed in various histologic-grade diseases.In cancer group,the most common genotypes were HPV16(80.8%),HPV33 (15.4%),HPV31(11.5%),HPV58(7.7%),HPV18(7.7%).HPV16 is the most popular genotype in all specimens,and more prevalent in cancer group and HSIL group compared with LSIL group and chronic cervieitis(P0.01).HPV68 wasn't detected in cancer group although it is main type in HSIL group and LSIL group.Low-risk genotype HPV6 was present in cancer group and HSIL group combined with other types of virus.Multiple-genotype infections were found in all cervical diseases,but single-type infection rate was increased with severity of the disease.Few HPV genotypes could be found solely existing in cancer group.Conclusions There exist geographical differences in the prevalence of HPV genotypes in cervical cancer.HPV16 remain the most common genotype in cervical cancer.Multiple-genotype infection can not increase the occurrence of cervical cancer.More studies are warranted to identify high-risk HPV genotypes.