Application of chitosan-gold Nanoparticles Hybrid Film Biosensor in Glucose Measurement

BACKGROUND: At present, syndromes happening frequency of diabetes mellitus can be controlled through regulating glucose concentration in blood. Therefore, a fast, steady, accurate glucose biosensor has been an intense area of focus for investigators. OBJECTIVE: To observe the effect of chitosan-gold nanoparticles hybrid film on electrochemical glucose biosensor. DESIGN, TIME AND SETTING: The observation experiment was performed at the Department of Medicine Laboratory, General Hospital, Guangzhou Military Command of Chinese PLA from February to April 2009. MATERIALS: Based on the immobilization of glucose oxidase (GOD) with cross-linking in the matrix of chitosan on an Au electrode by glutaraldehyde, a novel glucose biosensor was constructed with gold nanoparticles by electrodeposition. METHODS: The properties of CHIT/AuNPs hybrid film modified electrode were characterized by cyclic voltammetry (CV) in 20 mmol/L K3Fe (CN)6-0.1 mol/L KCI system by using the three-electrode system and the response to glucose was investigated by linear scan voltammetry (LSV). The optimal experiment conditions, stability and reproducibility about the glucose biosensor were also studied. MAIN OUTCOME MEASURES: The cyclic voltammetry properties of different modified electrode, as well as relation between glucose concentration and response current. RESULTS: Glucose concentration showed a linear correlation with response current in range of 0.001-6 mmol/L. The biosensor exhibited excellent performances of producibility, stability and the ignorance interferences of reducing substances, with the detection limited of 26.56μmol/L and fast response time (within 2 s). CONCLUSION: The glucose biosensor possesses excellent reproducibility and stability with a fast response time to glucose, which can be used in detection of glucose concentration in human serum.