A glucose biosensor based on glucose oxidase immobilized on chitosan/PVP composite membranes

A glucose biosensor was developed based on the immobilization of glucose oxidase in chitosan /PVP hybrid matrix. The electrochemical behavior of the new glucose biosensor was examined by cyclic voltammetry and the fabrication procedure was systematically optimized to improve the biosensor performance. The catalytic current response to glucose was linear in the range of 5. 0 × 10- 5- 8. 5 × 10- 4mol/L with a detection limit of 2. 0 × 10- 5mol/L( S/N = 3). The sensitivity of the biosensor for glucose was 5. 72 μA mmol/L and the apparent Michaelis-Menten constant( K m app) was calculated to be 2. 86 ×10- 3mol/L. The GOD biosensor has been used for the determination of glucose in real samples.