DIGFA for Detecting Serum Antibody IgG in Cases Infected with Pagumogonimus skrjabini

Objective To develop a simple, rapid and reliable assay for diagnosis of pagumogonimiasis skrjabini. Method Using Pagumogonimus skrjabini adult worm antigen and IgG lebelled with colloidal gold as color developing agent, a dot immunogold filtration assay (DIGFA) for detecting pagumogonimiasis skrjabini antibody IgG was established and Dot-ELISA was used as parallel control. Results There were no significant differences in positive rates between DIGFA (96.7%,88/91) and Dot-ELISA (92.3%,84/91) in 91 proved cases. The negative rate of DIGFA in healthy persons reached 100%(35/35). The cross rates of DIGFA in schistosomiasis and clonorchiasis cases were 4.0%(1/25) and 4.5%(1/22). The coincidence rate of DIGFA and Dot-ELISA reached 91.7%(44/48). Conclusion DIGFA, an assay with simplicity and without special equipment, was as sensitive and specific as Dot-ELISA.