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Cloning and sequence analysis of interleukin-17 gene of Guangxi Bama mini pig.

Xiong YanYun, Xie QiHui, Luo TingRong, LU Qin-zhang, Liao SuHuan

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Abstract

To amplify interleukin-17,using reverse transcription-polymerase chain reaction(RT-PCR) from the total RNA from Guangxi Bama mini pig's peripheral blood lymphocyte stimulated by ConA,the IL-17 gene was cloned into pMD18-T vector and identified by PCR and enzyme restriction analysis of recombinant IL-17. The sequence analysis showed that the nucleotide sequences of the IL-17 in the study shared 99.4 % homology with that of pig published in GenBank.

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What this paper is about

To amplify interleukin-17,using reverse transcription-polymerase chain reaction(RT-PCR) from the total RNA from Guangxi Bama mini pig's peripheral blood lymphocyte stimulated by ConA,the IL-17 gene was cloned into pMD18-T vector and identified by PCR and enzyme restriction analysis of recombinant IL-17. The sequence analysis showed that the nucleotide sequences of the IL-17 in the study shared 99.4 % homology with that of pig published in GenBank.

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Available abstract

To amplify interleukin-17,using reverse transcription-polymerase chain reaction(RT-PCR) from the total RNA from Guangxi Bama mini pig's peripheral blood lymphocyte stimulated by ConA,the IL-17 gene was cloned into pMD18-T vector and identified by PCR and enzyme restriction analysis of recombinant IL-17. The sequence analysis showed that the nucleotide sequences of the IL-17 in the study shared 99.4 % homology with that of pig published in GenBank.

Key concepts: GenBank, Biology, Sequence analysis, Cloning (programming), Gene, Molecular biology, Bama, Polymerase chain reaction

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Cloning and sequence analysis of interleukin-17 gene of Guangxi Bama mini pig. — Research Paper | ScholarLens