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Molecular cloning and sequencing of leptin gene mature peptides cDNA from Guangxi Bama mini-pig

Li Bai

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Abstract

The total RNA was purified from Guangxi Bama Mini-pig subcutaneous adipose tissue.A fragment with 441 bp was amplified by the Reverse Transcription Polymerase Chain Reaction(RT-PCR) and then was ligated into pMD18-T vector after purification.The recombinant was verified by the method of PCR,restrictive cleavage and sequencing,respectively.The result showed that the Bama Mini-pig leptin gene mature peptide cDNA was successfully cloned in this study.The homologous analysis indicated that the homologous rate was 99.06% compared with the porcine leptin gene reported in GenBank and this gene has high conservativeness among mammalian animals(84.5%).Four nonsence mutations were detected in leptin gene mature peptide cDNA sequence of Bama Mini-pig.

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What this paper is about

The total RNA was purified from Guangxi Bama Mini-pig subcutaneous adipose tissue.A fragment with 441 bp was amplified by the Reverse Transcription Polymerase Chain Reaction(RT-PCR) and then was ligated into pMD18-T vector after purification.The recombinant was verified by the method of PCR,restrictive cleavage and sequencing,respectively.The result showed that the Bama Mini-pig leptin gene mature peptide cDNA was successfully cloned in this study.The homologous analysis indicated that the homologous rate was 99.06% compared with the porcine leptin gene reported in GenBank and this gene has high conservativeness among mammalian animals(84.5%).Four nonsence mutations were detected in leptin gene mature peptide cDNA sequence of Bama Mini-pig.

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Available abstract

The total RNA was purified from Guangxi Bama Mini-pig subcutaneous adipose tissue.A fragment with 441 bp was amplified by the Reverse Transcription Polymerase Chain Reaction(RT-PCR) and then was ligated into pMD18-T vector after purification.The recombinant was verified by the method of PCR,restrictive cleavage and sequencing,respectively.The result showed that the Bama Mini-pig leptin gene mature peptide cDNA was successfully cloned in this study.The homologous analysis indicated that the homologous rate was 99.06% compared with the porcine leptin gene reported in GenBank and this gene has high conservativeness among mammalian animals(84.5%).Four nonsence mutations were detected in leptin gene mature peptide cDNA sequence of Bama Mini-pig.

Key concepts: Complementary DNA, Biology, Molecular biology, Gene, Cloning (programming), Molecular cloning, Bama, Recombinant DNA

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Molecular cloning and sequencing of leptin gene mature peptides cDNA from Guangxi Bama mini-pig — Research Paper | ScholarLens