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2004•Zhongguo shouyi ke-jiRequires access

Cloning and sequence analysis of porcine interleukin-18 gene

Zhizhong Jing, Yongxi Dou, Junlin Hou, Meng Xue-lian, Xuepeng Cai

Open publisher page 2 citations

Abstract

After porcine PBMC was stimulated by LPS and PHA, pIL-18 gene was amplified by reverse transcription and polymerase chain reaction (RT-PCR) in the present study. The sequence analysis showed that the nucleotide sequence of the pIL-18 gene cloned in the study shares 100% homology with that published in GenBank and differences significantly from human, mouse, cow, chicken, dog, horse, rat, sheep and turkey IL-18 gene’s sequences, which were published in GenBank.

About this research paper

What this paper is about

After porcine PBMC was stimulated by LPS and PHA, pIL-18 gene was amplified by reverse transcription and polymerase chain reaction (RT-PCR) in the present study. The sequence analysis showed that the nucleotide sequence of the pIL-18 gene cloned in the study shares 100% homology with that published in GenBank and differences significantly from human, mouse, cow, chicken, dog, horse, rat, sheep and turkey IL-18 gene’s sequences, which were published in GenBank.

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Available abstract

After porcine PBMC was stimulated by LPS and PHA, pIL-18 gene was amplified by reverse transcription and polymerase chain reaction (RT-PCR) in the present study. The sequence analysis showed that the nucleotide sequence of the pIL-18 gene cloned in the study shares 100% homology with that published in GenBank and differences significantly from human, mouse, cow, chicken, dog, horse, rat, sheep and turkey IL-18 gene’s sequences, which were published in GenBank.

Key concepts: GenBank, Biology, Gene, Homology (biology), Cloning (programming), Sequence analysis, Polymerase chain reaction, Molecular biology

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