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2013•Journal of Liquid Chromatography & Related TechnologiesRequires access

ISOLATION AND PURIFICATION OF FORSYTHOSIDE A AND SUSPENSASIDE A FROM FORSYTHIA SUSPENSA BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY

Mei Yang, Xinjun Xu, Chunyan Xie, Jieyun Huang, Zhisheng Xie, Depo Yang

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Abstract

Forsythoside A and suspensaside A were isolated and purified from Forsythia suspensa by one step of high-speed counter-current chromatography for the first time using a solvent system of ethyl acetate-n-butanol-methanol-water (4:0.5:0.5:5, v/v). The purities of the two compounds were 98.19% and 98.05%, respectively, as determined by HPLC. Their structures were identified by MS, UV, and NMR analysis. Such a simple and effective method was fairly useful to prepare pure compounds as reference substances for related study on Forsythia suspensa.

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What this paper is about

Forsythoside A and suspensaside A were isolated and purified from Forsythia suspensa by one step of high-speed counter-current chromatography for the first time using a solvent system of ethyl acetate-n-butanol-methanol-water (4:0.5:0.5:5, v/v). The purities of the two compounds were 98.19% and 98.05%, respectively, as determined by HPLC. Their structures were identified by MS, UV, and NMR analysis. Such a simple and effective method was fairly useful to prepare pure compounds as reference substances for related study on Forsythia suspensa.

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Available abstract

Forsythoside A and suspensaside A were isolated and purified from Forsythia suspensa by one step of high-speed counter-current chromatography for the first time using a solvent system of ethyl acetate-n-butanol-methanol-water (4:0.5:0.5:5, v/v). The purities of the two compounds were 98.19% and 98.05%, respectively, as determined by HPLC. Their structures were identified by MS, UV, and NMR analysis. Such a simple and effective method was fairly useful to prepare pure compounds as reference substances for related study on Forsythia suspensa.

Key concepts: Chemistry, Forsythia, Chromatography, Countercurrent chromatography, High-performance liquid chromatography, Ethyl acetate, Methanol, Organic chemistry

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ISOLATION AND PURIFICATION OF FORSYTHOSIDE A AND SUSPENSASIDE A FROM FORSYTHIA SUSPENSA BY HIGH-SPEED COUNTER-CURRENT CHROMATOGRAPHY — Research Paper | ScholarLens