Experimental Study on Inhibitory Effect of Forsythia Extract on Inflammatory Response Induced by Endotoxin

Objective:To experimentally study the inhibitory effect of Forsythia extract on inflammatory reˉsponse induced by endotoxin.Methods:(1)The Forsythia extract was produced first,and then it was diluted to different concentrations.These diluted agents were mixed with identical endotoxin,and the concentrations of endotoxin in mixtures were detected after several minutes.(2)The rat models of peritonitis caused by Esˉcherichia coli were treated by Gentamicine,Forsythia extract and Gentamicine plus Forsythia extract respecˉtively.The levels of IL_1and TNF_αin plasma and the results of in situ hybridization of IL_1and TNF_αmRNA of liver tissue were observed.Results:When the concentration of Forsythia extract was above31.25g/L,it can inhibit endotoxin markedly.The levels of IL_1and TNF_αof plasma and liver tissue in Forsythia group and Gentamicine plus Forsythia group were lower than those in Gentamicine group and peritonitis group,but there was no difference between the Forsythia group and the Gentamicine plus Forsythia group.Conclusion:The Forsythia extract can be against endotoxin and inhibit the overexpression of cytokines induced by endotoxin evidently.Its effect of against endotoxin is superior to Gentamicine.When the Forsythia extract is combined with Gentamicine,its effect is not enhanced.