HPLC Analysis for Simultaneous Determination of Rabeprazole and Domperidone in Pharmaceutical Formulation

A simple and sensitive reversed phase high performance liquid chromatographic (RP‐HPLC) method has been developed for the quantitative estimation of rabeprazole and domperidone in their combined dosage forms. Rabeprazole and domperidone were chromatographed using 0.01 M, pH 6.5 ammonium acetate buffer:methanol:acetonitrile (40:30:30 v/v, pH 7.44) as the mobile phase at a flow rate of 1.0 mL min−1 at ambient temperature and detected at 287 nm. The retention time (RT) of rabeprazole and domperidone were found to be 6.13±0.01 and 8.38±0.02, respectively. The linearities of rabeprazole and domperidone were in the range of 200–2000 ng/mL and 300–3000 ng/mL, respectively. The limit of detection was found to be 65.67 ng/mL for rabeprazole and 98.33 ng/mL for domperidone. The proposed method was applied for the determination of rabeprazole and domperidone in combined dosage forms.