Evaluation of the animal model of hepatic fibrosis caused by schistosomiasis.

Objective To establish and evaluate animal model of hepatic fibrosis caused by schistosomiaisis.Methods 34 rabbits,each was challenged by 100 S.j cercariae,were induced to hepatic fibrosis.12～13 weeks post challenge,liver-clearing oral medicine(DHT) and praziquantel(PQT) were adopted as intervene therapies.Those rabbits with hepatic fibrosis were distributed into 3 groups randomly:high dose group(group A);low dose group(group B) and control group(group C).The pathology of hepatic tissue and HYP HA levels in serum were served as index of model evaluation.Results 12～13 weeks post challenge,the animal model of hepatic fibrosis was established.The pathology and HYP /HA level in serum were changed in different degrees.The HYP /HA levels in serum of A,B groups pre and post therapy intervention showed a significant decrease(P0.01).And the results of pathology of liver tissues and HYP /HA levels showed that the degrees of hepatic fibrosis and serum levels of HYP /HA in high dose grouplow dose groupcontrol group(P0.01).Conclusion The detection of the changes of HYP /HA levels in serum is a convenient,rapid,reliable and noninvasive index in evaluating the therapeutic effect on hepatic fibrosis and also the changes of HYP /HA level in serum are correspond with the results of pathology,so the detection of those indices has a pragmatic value in application.