2011•Dongbei Nongye Daxue xuebaoRequires access
Construction of DNA fingerprinting of four tomato varieties and their parents
Pan Lu, Kang LiGong, Xiangyang Xu, Jingfu Li
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Abstract
Total genomic DNA of four tomato varieties and their parents was extracted by using CTAB method.DNA fingerprinting maps of four tomato varieties and their parents were established by AFLP-PCR method.The results showed that nine primers from 20 primers were selected.The amplified polymorphic bands of the parents were from 10 to 27,and the average bands was 17.56,the percentage of polymorphic bands was 41.25%.The purity of seven parents was 100%,and that of the tomato varieties was 98.7%.The genetic similarity coefficients of four varieties and their parents were from 0.48 to 0.71 and from 0.62 to 0.76.The cluster analysis was conducted and the genetic similarity coefficient was calculated by using UPGMA method.This study will provide the theory basis for tomato cross breeding and cultivars identification.