AFLP Analysis of New Pear Cultivars and Their Parents

Forty-three pear cultivars including 20 new pear cultivars and their parents were subjected to fluorescent AFLP (amplified fragments length polymorphism) analysis. Seven primer combinations selected from 64 primer combinations produced a total of 784 fragments, of which 699 were polymorphic with a polymorphism percentage of 89.2%. The results showed that characteristic bands were amplified from 29 cultivars, and any primer combination could identify all the cultivars. Fluorescent AFLP was proved to be a very reliable method to identify pear cultivars. The genetic relationship between new pear cultivars and their parents was analyzed and 20 new cultivars were classified on molecular level by clustered analysis, which provided theoretical reference for parent selection in pear hybridization breeding.