Preliminary study on the tissue culture and rapid propagation system of Cyclocarya paliurus.

Using mature embryo of Cyclocarya paliurus as experimental materials,we preliminarily established the tissue culture and rapid propagation system in this experiment.The result indicated that the seeding rate of excised embryos was higher in culture medium with 30 g/L sucrose and 6.5 g/L agar for 60 days,which reached 72.67%.Through orthogonal test,the optimal culture medium for multiple shoots induction was composed of WPM+0.5 mg/L 6-BA+0.01 mg/L IBA+30 g/L sucrose and the suitable subculture cycle was 40 days,in which the induction rate of multiple shoots was 93.33% and the highest multiplication coefficient was 4.75.The influence order of the three kinds of hormones on multiplication coefficient of multiple shoots was IBA6-BAKT,which of IBA had significant effect.Uniconazole and rare earth lanthanum in culture medium had positive effect on strong seeding and growth of plantlet.The optimal concentrations of uniconazole and rare earth lanthanum were 0.1 mg/L and 5 mg/L,respectively.The single factor experiment showed that the suitable concentration of IBA played a certain role for rooting of seeding in vitro,and the rooting rate was 16.67% in medium containing WPM+0.2 mg/L IBA+20 mg/L sucrose.After 15 days dark induction in this medium,the rooting rate could be increased to 23.33%.