Multiple Shoots Induction and Rapid Propagation of Lonicera Edulis

Sprouts being explants, the factors affecting in vitro culture and plant regeneration of L. edulis were optimized by orthogonal design and tissue cultured seedlings were obtained on culture media with different concentrations of hormones.The results showed that the optimum compositions for primary culture, subculture and rooting culture were MS+6-BA 1.0 mg/L + IBA 0.2 mg/L, MS+6-BA 0.5 mg/L +IBA 0.3 mg/L +KT 1.5 mg/L and 1/4MS+IBA 1.5 mg/L.The differentiation rate, proliferation coefficient and rooting rate on these media were up to 100%, 8.7, and 96.7% respectively.The combination of 6-BA, IBA and KT was effective in the number of multiple shoots of L. edulis in subculture.The micro-propagation technology introduced to cultivate the tissue cultured seedlings proved to meet the need of rapid propagation of plantlets and could be applied in mass propagation.