Studies on the technique system of tissue culture and rapid propagation of Jojoba II.various hormone combinations in the differentiation and rooting culture

Studies on hormone combinations of 3 kinds of cultures including primary culture, subculture and rooting culture in the micropropagation method of jojoba showed that the axillary buds of nodal segments were induced to proliferation on modified MS medium supplemented with 2 mg/L 6-BA, 0.5 mg/L GA_3 and 0.05 mg/L IBA.On an average, 4.9 shoots were formed per original explant in the initial cultures.The number of shoots in subsequent culture reached 7.8 on 1 mg/L 6-BA-containing medium. If using the combination of 2 mg/L6-BA and 0.5 mg/L GA_3,the multiplication coefficient reached 8.3. It indicated that 6-BA combining with low concentration of GA_3 promoted the shoots propagation.6-BA shouldn't exceed 3 mg/L, because high concentration of cytokinin enhanced the ratio of hyper hydricity.After culture for 20 days on the 1/2 modified MS medium supplemented with 3 mg/L IBA, 1.5 mg/L NAA and 0.5 mg/L IAA, about 83% of the shoots had developed roots.