2009•Acta Agriculturae ZhejiangensisRequires access
Rapid propagation in vitro of jasmine.
Sun YanNi, Tang FangPing, Weimin Fang, Tan Guo-hua
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Abstract
The effects of different concentrations of 6-BA and IBA on induction of bud germination,and proliferation of jasmine at different temperatures were studied.Moreover,effects of different concentrations of NAA and rooting culture modes on rooting were investigated.Results showed that the highest bud induction rate of 96.75% was obtained in the WPM medium supplemented with 2.0 mg/L 6-BA and 0.1 mg/L IBA.The best proliferation with a shoot proliferation coefficient of 5.31 was observed in the WPM medium supplemented with 1.0 mg/L 6-BA and 0.2 mg/L IBA at 35℃.The optimal rooting culture mode was two-step rooting method,i.e.,pre-culturing shoots on the rooting medium of 1/2WPM+NAA 1.0 mg/L for 7 days,then transferring pre-cultured shoots to 1/2WPM without supplement of any plant growth regulators.The rooting rate was up to 98.41%.