Cloning and differential expression of SR-BIgene in pigs

【Objective】 cDNA sequences of the SR-BⅠ gene were obtained and analyzed in biologic information,based on which the expression of SR-BⅠ in pigs was studied.【Method】 Visceral fat,subcutaneous fat,liver,spleen,muscle,kidney,lung and heart of different growth-stages and different types of pigs were collected.A pair of PCR primers were designed and synthesized according to SR-BⅠ gene sequence of human,mouse and rat.Total RNA was extracted from adipose tissue of pigs,and then the SR-BⅠ gene cDNA was cloned by RT-PCR.Thereafter,the level of SR-BⅠ mRNA in various regions,different growth-stage,different parts and different types of pig adipose tissue were measured.【Result】 The sequenceing results showed that 192 bp pig's SR-BⅠ cDNA fragment(EU366320) was successfully cloned.Homologous comparison with some animals indicated that pig SR-BⅠ cDNA shared 84.1%,78.6%,77.5%,67.0%,66.6% similarity in bovine,human,cricetulus griseus,mouse and rat.The expression level of SR-BⅠ mRNA was significantly higher in adipose tissue of lean pigs than that of obese pigs,and also the expression level gradually increased as age grew.It was found that the abundance of SR-BⅠ mRNA level increased more in subcutaneous fat than in visceral fat.【Conclusion】 Our result suggested that SR-BⅠ is a potential factor which may regulate adipogenesis in adipose tissue.