2011•Xumu shouyi xuebaoRequires access
Expression Analysis of DECR1 Gene in Tissues of Mashen and Large White Pig
Zhou Zhong-xiao
Open publisher page 1 citations
Abstract
The purpose of this study was to analyze DECR1 mRNA and protein expression profiles of Mashen and Large White pigs in different tissues,and evaluate the potential relationships between its relative expression and lipid metabolism.DECR1 expression profiles in nine tissues(liver,kidney,spleen,lung,small intestine,subcutaneous adipose,stomach,longissimus dorsi,heart) of Mashen and Large White pigs were examined by quantitative real-time PCR and Western blot,respectively.The results indicated that,by real-time PCR,DECR1 had the tissue-and species-specific in mRNA expression,but highly in liver,subcutaneous adipose and heart.The DECR1 mRNA expression in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig(P0.01).The results showed that,by Western blot,the distribution of DECR1 in tissues were significantly different,but highly in subcutaneous adipose,liver and small intestine.Moreover,the expression of DECR1 in subcutaneous adipose of Large White pig was significantly higher than that of Mashen pig(P0.05).It was inferred that DECR1 gene may be related to regulatory mechanism of lipid metabolism.