TISSUE EXPRESSION PROFILES OF GPx4 GENE IN LAIWU PIGS

The purpose of this investigation was to analyze GPx4 gene mRNA expression profiles of Laiwu pigs,and to evaluate the potential relationships between GPx4 mRNA relative expression and antioxidative capacity of Chinese native pigs.Five Laiwu pigs were used as experimental animal,and total RNA was isolated from 10 tissues of Laiwu pig.The mRNA expression levels of GPx4 gene of different tissues were measured using quantitative real-time PCR,and the results showed that it was tissue specific in GPx4 mRNA expression and the levels of mRNA expression were significantly difference between tissues.The GPx4 mRNA expression levels of brain,heart,liver and spleen were significantly higher than that of the other tissues(P0.01).The rank order of the GPx4 mRNA expression in the other tissues was muscle spinal cord,large intestine,backfat and small intestine lung,and there were significantly different(P0.05).