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Determination of the total flavonoids extracts of RADIX PUERARIAE by HPLC.

Zhuang Xiao-mei, Han Shu-qin, LI Zhi-rui

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Abstract

[Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25∶75, V/V), detection wavelength of 250 nm, flow speed of 0.8 ml/min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg/ml, respectively. [Conclusion] This research provided basis for better utilization of RADIX PUERARIAE.

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What this paper is about

[Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25∶75, V/V), detection wavelength of 250 nm, flow speed of 0.8 ml/min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg/ml, respectively. [Conclusion] This research provided basis for better utilization of RADIX PUERARIAE.

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Available abstract

[Objective] The aim was to determine the puerarin and daidzin contents in total flavonoids extracts of RADIX PUERARIAE. [Method] HPLC conditions were: chromatographic column of C18 column, mobile phase of acetonitrile-water (25∶75, V/V), detection wavelength of 250 nm, flow speed of 0.8 ml/min, column temperature of room temperature and sampling amount of 4 μl. [Results] Within the determination range, puerarin and daidzin control substances showed good linear relationship. And The puerarin and daiazin contents in total flavonoids of RADIX PUERARIAE were 5.41 and 7.50 μg/ml, respectively. [Conclusion] This research provided basis for better utilization of RADIX PUERARIAE.

Key concepts: Puerarin, Daidzin, Chromatography, Radix (gastropod), Chemistry, High-performance liquid chromatography, Botany, Daidzein

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Determination of the total flavonoids extracts of RADIX PUERARIAE by HPLC. — Research Paper | ScholarLens