1995•The Korean Journal of MycologyRequires access

Standardization of Chemically Defined Medium for the Production of Mycelium and Basidiocarps in Flammulina velutipes

Chi-Hyeun Song, Chang-Ho Lee, Jang‐Hyuk Ahn, Bum-Shik Hong, Han-Chul Yang

Abstract

Nutritional requirements for the growth of Flammulina velutipes were studied. Mannitol, glutamic acid and ammonium nitrate were chosen for the maximum mycelial growth when various carbon and nitrogen sources tested. Optimum C : N ratio for the mycelial growth was 20 : 1. Potassium dihydrogen phosphate was selected among the phosphate sources. Magnesium sulphate and thiamine HCl stimulate mycelial growth. Final compositions of optimized chemically defined medium were 1.5% mannitol, 0.082% , 0.312% glutamic acid, 0.25% , 0.06% thiamin HCl. This medium not only support mycelial growth but also induce fruit body formation.

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Key concepts: Flammulina, Mycelium, Mannitol, Basidiocarp, Chemistry, Ammonium phosphate, Food science, Ammonium