Study on the Optimization of Liquid Cultural Medium of Flammulina velutipes

Taking Flammulina velutipes as material,the factors influencing mycelium growth were screened in order to determine the best liquid strain culture medium of Flammulina velutipes that produce intracellular polysacchride.The dry weight of the mycelia and the number of mycelium pellet were selected as the investigation index for determining the carbon source,nitrogen source,inorganic salt and vitamin.The fermentation test and the orthogonal test were used to analysis the four factors and to determine the optimum culture medium of liquid strain of F.velutipes.The results showed that the optimum carbon source for liquid culture of F.velutipes was soluble starch,the optimum nitrogen source was soybean flour,the optimum inorganic salt was KH2PO4,and the optimum vitamin was VB2,and the optimum formula of culture medium was as follows:4%soluble starch,5%soybean flour,0.05% KH2 PO4,0.0075% VB2.