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[Preliminary studies on microwave irradiation ELISA for diagnosis of Schistosomiasis japonica].

Q Gu, Hongtao Zhang, Shouyue Zhu, Wen Rong Xu

Abstract

AIM: To explore a fast and highly efficient method for the diagnosis of schistosomiasis japonica. METHODS: Using microwave irradiation ELISA (MWI-ELISA) and fast-ELISA to detect specific antibodies in sera from 118 cases with schistosomiasis japonica, 61 healthy individuals and 12 paragonimiasis cases. RESULTS: The positive rates of schistosomiasis cases were 88.1% (104/118) by MWI-ELISA and 91.5% (108/118) by fast-ELISA, respectively (chi 2 = 0.74, P > 0.05). The false positive reaction of healthy individuals was found in 2 cases(3.3%) by MWI-ELISA and 1 case (1.6%) by fast-ELISA, respectively (chi 2 = 0.34, P > 0.05). No false positive reaction was found in paragonimiasis cases. CONCLUSION: The sensitivity and specificity of the two tests were similar, however, MWI-ELISA was faster than fast-ELISA.

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Available abstract

Key concepts: Paragonimiasis, SCHISTOSOMIASIS JAPONICA, Schistosomiasis, Medicine, Microwave irradiation, Antibody, Japonica, Gastroenterology