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[Circulating antigen detection in schistosomiasis japonica patients by sandwich ELISA using polyclonal and monoclonal antibodies].

L S Qiu, Ziding Feng, Y H Zhang, Hong Li

Abstract

OBJECTIVE: To detect circulating antigen in the patients with schistosomiasis. METHODS: Polyclonal antibody(PcAb)-monoclonal antibody(McAb) sandwich ELISA. RESULTS: Sera from 150 patients with schistosomiasis were detected, the average sensitivity was 84.7%(72.0%-91.0%). No false positive reaction was detected in 40 normal controls and none but 2 patients(2/10) with paragonimiasis showed cross reactions among 74 cases with other parasitic infections. CONCLUSION: PcAb-McAb sandwich ELISA is a relatively ideal method for circulatling antigen detection.

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Available abstract

Key concepts: Polyclonal antibodies, Paragonimiasis, Schistosomiasis, SCHISTOSOMIASIS JAPONICA, Monoclonal antibody, Antigen, Antibody, Monoclonal