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A Study on the Extraction Efficiency of Berberine in Coptidis Rhizoma

Jae Seong Rhee, Ho Koon Park, Eun Ran Woo, Eun Ju Lee, Dug Kyun Ahn, Won Sik Youn, Kwang Ho Shin

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Abstract

In this paper, optimum extraction condition for berberine which is the indan companent of Coptidis rhizoma was examined at various conditions in the aspect of temperature, solvent and time followed by quantitation with reversed phase HPLC. The efficiency for extraction has been shown the best at with 2~3 hours reflux time in methanol or acetic acid. Any significant effect can not be shown from the addition of SLS to extraction solvent.

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What this paper is about

In this paper, optimum extraction condition for berberine which is the indan companent of Coptidis rhizoma was examined at various conditions in the aspect of temperature, solvent and time followed by quantitation with reversed phase HPLC. The efficiency for extraction has been shown the best at with 2~3 hours reflux time in methanol or acetic acid. Any significant effect can not be shown from the addition of SLS to extraction solvent.

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Available abstract

In this paper, optimum extraction condition for berberine which is the indan companent of Coptidis rhizoma was examined at various conditions in the aspect of temperature, solvent and time followed by quantitation with reversed phase HPLC. The efficiency for extraction has been shown the best at with 2~3 hours reflux time in methanol or acetic acid. Any significant effect can not be shown from the addition of SLS to extraction solvent.

Key concepts: Berberine, Acetic acid, Extraction (chemistry), Chromatography, Chemistry, Solvent, Methanol, High-performance liquid chromatography

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