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1986•FEMS Microbiology LettersOpen access

Type II restriction endonucleases from Bacillus sphaericus

Rosanne Mullings

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Abstract

We report the isolation and characterisation of 3 Type II restriction endonucleases from Bacillus sphaericus. These are BspAI (an isoschizomer of Sau3AI) in strain JL4B; BspBI and BspBII (isoschizomers of PstI and AsuI) in strain JL14. These are the first reports of these activities in B. sphaericus and the first citation of an AsuI isoschizomer in a member of the genus Bacillus. We briefly discuss the possible uses in vitro and significance in vivo of these enzymes.

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We report the isolation and characterisation of 3 Type II restriction endonucleases from Bacillus sphaericus. These are BspAI (an isoschizomer of Sau3AI) in strain JL4B; BspBI and BspBII (isoschizomers of PstI and AsuI) in strain JL14. These are the first reports of these activities in B. sphaericus and the first citation of an AsuI isoschizomer in a member of the genus Bacillus. We briefly discuss the possible uses in vitro and significance in vivo of these enzymes.

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Available abstract

We report the isolation and characterisation of 3 Type II restriction endonucleases from Bacillus sphaericus. These are BspAI (an isoschizomer of Sau3AI) in strain JL4B; BspBI and BspBII (isoschizomers of PstI and AsuI) in strain JL14. These are the first reports of these activities in B. sphaericus and the first citation of an AsuI isoschizomer in a member of the genus Bacillus. We briefly discuss the possible uses in vitro and significance in vivo of these enzymes.

Key concepts: Bacillus sphaericus, Isoschizomer, Restriction enzyme, Biology, Bacillus (shape), PstI, Bacillaceae, Microbiology

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