ScholarLens Back to search
1986•FEMS Microbiology LettersRequires access

Type II restriction endonucleases fromBacillus sphaericus

Rosanne Mullings, Leslie Evans, Nigel L. Brown

Open publisher page 2 citations

Abstract

We report the isolation and characterisation of 3 Type II restriction endonucleases from Bacillus sphaericus. These are BspAI (an isoschizomer of Sau3AI) in strain JL4B; BspBI and BspBII (isoschizomers of PstI and AsuI) in strain JL14. These are the first reports of these activities in B. sphaericus and the first citation of an AsuI isoschizomer in a member of the genus Bacillus. We briefly discuss the possible uses in vitro and significance in vivo of these enzymes.

About this research paper

What this paper is about

We report the isolation and characterisation of 3 Type II restriction endonucleases from Bacillus sphaericus. These are BspAI (an isoschizomer of Sau3AI) in strain JL4B; BspBI and BspBII (isoschizomers of PstI and AsuI) in strain JL14. These are the first reports of these activities in B. sphaericus and the first citation of an AsuI isoschizomer in a member of the genus Bacillus. We briefly discuss the possible uses in vitro and significance in vivo of these enzymes.

Why it matters

OpenAlex reports 2 citations for this work. Citation counts describe recorded attention and do not establish research quality.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

We report the isolation and characterisation of 3 Type II restriction endonucleases from Bacillus sphaericus. These are BspAI (an isoschizomer of Sau3AI) in strain JL4B; BspBI and BspBII (isoschizomers of PstI and AsuI) in strain JL14. These are the first reports of these activities in B. sphaericus and the first citation of an AsuI isoschizomer in a member of the genus Bacillus. We briefly discuss the possible uses in vitro and significance in vivo of these enzymes.

Key concepts: Bacillus sphaericus, Isoschizomer, Restriction enzyme, Biology, Bacillus (shape), Bacillaceae, Bacillus pumilus, PstI

Related papers

Related paper 1Related paper 2
Back to paper searchBrowse research topicsOriginal source
Type II restriction endonucleases fromBacillus sphaericus — Research Paper | ScholarLens