Optimization of Extraction Process of Ursolic Acid from FRUCTUS CHAENOMELIS and Determination of Its Content

[Objective] To optimize the extracting conditions of ursolic acid from FRUCTUS CHAENOMELIS and determine its content,so as to provide scientific evidence for the development,application and quality control of ursolic acid.[Method] The extraction conditions of ursolic acid were optimized by orthogonal test,and content of ursolic acid was determined by HPLC.[Result] The optimal extraction conditions were determined to be: 30 min of extraction duration,25.0 ml of ethanol amount and extraction solvent of absolute ethanol.Under the optimum process,content of ursolic acid was 0.324%.When the concentration of ursolic acid ranged from 0.650 to 3.250 μg/ml,the relationship between ursolic acid concentration and peak area was good(r=0.999 1).Mean recovery rate of ursolic acid was 99.20% with RSD value of 0.30%.[Conclusion] The optimal extraction conditions were feasible,and the established HPLC method was simple,sensitive and accurate.This study provided scientific evidence for the development,application and quality control of ursolic acid.