2009Xiandai shipin kejiRequires access

Determination of Total Flavonoids Content in Citrus Peels by Ultraviolet Spectrophotometry

LV Lin, Ningping Tao

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Abstract

A simple and accurate method for the determination of the total flavonoids content in citrus peels was developed. A comparison was made for the determination of the content of total flavonoids in Citrus peels by ultraviolet spectrophotometry at 310 nm using rutin as the standard compound and by the spectrophotometry in the NaNO2-Al(N03)3-NaOH system at 510 nm. Results showed that,with ultraviolet spectrophotometry at 310 nm,good linearity of Rutin concentration was found within the range of 10~50 mg/L(R2=0.9996) with the average recovery rate and RSD being of 99.68% and 1.20%(n=6),respectively. The method is simple and accurate,which can be applied to determine total flavonoids in citrus peels.

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What this paper is about

A simple and accurate method for the determination of the total flavonoids content in citrus peels was developed. A comparison was made for the determination of the content of total flavonoids in Citrus peels by ultraviolet spectrophotometry at 310 nm using rutin as the standard compound and by the spectrophotometry in the NaNO2-Al(N03)3-NaOH system at 510 nm. Results showed that,with ultraviolet spectrophotometry at 310 nm,good linearity of Rutin concentration was found within the range of 10~50 mg/L(R2=0.9996) with the average recovery rate and RSD being of 99.68% and 1.20%(n=6),respectively. The method is simple and accurate,which can be applied to determine total flavonoids in citrus peels.

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Available abstract

A simple and accurate method for the determination of the total flavonoids content in citrus peels was developed. A comparison was made for the determination of the content of total flavonoids in Citrus peels by ultraviolet spectrophotometry at 310 nm using rutin as the standard compound and by the spectrophotometry in the NaNO2-Al(N03)3-NaOH system at 510 nm. Results showed that,with ultraviolet spectrophotometry at 310 nm,good linearity of Rutin concentration was found within the range of 10~50 mg/L(R2=0.9996) with the average recovery rate and RSD being of 99.68% and 1.20%(n=6),respectively. The method is simple and accurate,which can be applied to determine total flavonoids in citrus peels.

Key concepts: Rutin, Spectrophotometry, Chemistry, Ultraviolet, Chromatography, Recovery rate, Flavonoid, Biochemistry

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