Cytotoxicity effect of nonpolar extract from parijoto (medinilla speciosa reinw. ex. bl) fruit against hela and widr cell line

Abstract Cisplatin is a chemotherapy agent in the treatment of cervical cancer. However, due to drug resistance and its side effects are much needed an agent that can be combined with cisplatin. Parijoto fruit (Medinilla speciosa Reinw.ex.Bl) have potential cytotoxic effect derived from natural ingredients. The study aims to determine the potential cytotoxic effects of ethyl acetate and n-hexane extract from Parijoto fruit calculated from the value of IC50 and the profile of these extract on normal cell. The determination of the cytotoxic effect of Parijoto fruit extract using MTT assay that the results can be read absorption by using ELISA reader. Data analysis is calculated by linear regression methods by using Microsoft Excel software. Results showed that ethyl acetate and n-hexane of parijoto fruit performed cytotoxic effect on HeLa cell line with IC50 respectively, 352,9 μg/mL; 904,7 μg/mL while the value of ethyl acetate and n-hexane of parijoto fruit performed cytotoxic effect on WiDr cell line with IC50 respectively, 554,9 μg/mL; 434,4 μg/mL. Data analysis showed that the cytotoxicity effect of nonpolar extract from parijoto fruit is include in the moderate cytotoxic category.