Effect of apoptosis rate on cervical carcinoma cell line HeLa by 4-HPR and Cisplatin

Objective To investigate the apoptosis inducing effect of N-4-hydroxyphenyl retinode(4-HPR)and Cisplatin on human HeLa cell lines.Methods MTT assay was employed to examine the growth suppression of the cells.TEM was used to observe the ultrastructural change of HeLa cells treated with 4-HPR and Cisplatin.The apoptosis rate and change in cell cycle of HeLa cells treated with 4-HPR or/and Cisplatin were examined by flow cytometer.Results 4-HPR and Cisplatin both could induce apoptosis of HeLa cells;combined use of 4-HPR and Cisplatin could enhance HeLa cells apoptosis.Conclusion 4-HPR and Cisplatin could induce apoptosis of HeLa cells,and their effect is synergetic.