Separation and Purification Process of Total Flavonoids from Elaeagnus mollis Leaves

The content of total flavonoids in Elaeagnus mollis Diels leaves was determined by ultraviolet spectrophotometry,the adsorption capacity and desorption rate of four kinds of macroporous resins(D4020、AB-8、S-8、X-5) were calculated to determine the suitable resin and its optimum process conditions. The results indicated: S-8 resin had the highest adsorption capacity(9.83mg·g-1), followed by AB-8(9.38mg·g-1), but the desorption rate of AB-8 resin(88.79%) was better than that of S-8 resin(71.89%).AB-8 resin was the best absorbent for the total flavonoids in E.mollis leaves. The optimum process conditions were: the pH value of extracts was 3.0~4.0, the ratio of absorbing solution to resin was 6∶1, the adsorption flow rate was 2mL·min-1, the eluting solution was 60% ethanol, the eluting flow rate was 2mL·min-1, the volume of the eluting solution was 3BV.