Study on the Separation and Purification Process of Flavonoids from Elaeagnus mollis Diels Leaves with Polyamide Resin

The best purification technology of the flavonoids from Elaeagnus mollis Diels leaves with polyamide resin was study in this article.The optimum purification technique was confirmed by studying the static and dynamic absorption and desorption of flavonoids from Elaeagnus mollis Diels leaves with two kinds of polyamide resins(60-100,100-200) under various conditions.The results indicated the polyamide resin(100-200) was the best absorbent of the flavonoids in Elaeagnus mollis leaves.Saturated adsorption was 0.153 mg/g,the desorption rate was 85.88%.The optimum technical conditions were that the pH of extracts was 4.0-6.0,the eluting reagent was 60% ethanol,the best ratio of absorbing solution and resin was 6∶1,the volume of the eluting solution was 1.5 BV,the polyamide resin can be used 4-5 times repeatedly.