Study on the Enrichment Technology of Total Flavonoids in Elaeagnus Angustifolia Flower by Macroporous Adsorption Resin

OBJECTIVE To screen the best macroporous adsorptive resin for separation and purification of flower Elaeagnus angustifolia flavonoids and to establish the purification process parameters.METHODS Using macroporous adsorption resin adsorption rate and desorption rate as the indexes,the best resin was screened out for enriching flower Elaeagnus angustifolia flavonoids.The optimal process conditions were investigated through single factor test.RESULTS DA201 resin showed good adsorption and separation property for Elaeagnus angustifolia flavonoids.The detailed process conditions were as follows: the ratio of diameter to height of resin column was 1∶8,sample loading rate was 0.5 mL·min-1,sample concentration was 20 mg·mL-1,pH was adjusted to 4,with elution rate of 0.5 mL·min-1,the largest sample load was 4.5 BV,eluent concentration was 80% and the dosage was 12 BV.CONCLUSION DA201 type resin,showed good purification effect for flavonoids Elaeagnus angustifolia in the defined process conditions with a yield of 85.23%.The purity of total flavonoids achieved 14.95%,which was 3 times of that by coarse extraction(4.901%).