Separation and Purification of Three Xanthones from Swertia Mussotti Franch by High Speed Counter-Current Chromatography

High speed counter-current chromatographic(HSCCC) method was used to purify and separate methylswertianin,gentiacaulein and decussatin from Swertia mussotti Franch.with a two-phase solvent system composed of hexane-ethyl acetate-ethanol-water(5∶5∶6∶4,V/V).The upper phase was used as the stationary phase and the lower phase was used as the mobile phase at a flow rate of 2.5 mL/min.The experimental conditions were controlled at 850 r/min and 25 ℃,and the effluent was detected at 254 nm.The separation producs of methylswertianin,gentiacaulein and decussatin with the purity of over 98% were obtained by high performance liquid chromatography,and identification of their structures was performed by 1H NMR,13C NMR and 2D NMR.