2006•Zhonghua wei-chang waike zazhiRequires access
Application of proteomics in the research of discrepancy proteins in gastric cancer
Ding Da-yon
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Abstract
Objective To explore the discrepancy proteins in gastric cancer by proteome analysis. Methods Total proteins of gastric cancer tissues and matched normal gastric epithelial tissues were separated respectively by two-dimensional polyacrylamide gel electrophoresis (2-DE).Mass spectrometry was used to test the differentially expressed proteins.Results One thousand one hundred and forty-seven protein spots from gastric cancer tissue and 1079 spots from the normal tissue were gained.Out of 164 different protein spots,41 were only expressed in gastric cancer tissue,27 were unique in normal tissue,39 were up-regulated and 57 were down-regulated in gastric cancer.Seven proteins,which were highly expressed in gastric cancer tissue,were identified.Conclusion Different protein spots between gastric cancer tissues and normal gastric epithelial tissue were gained by proteomics.The 7 discrepancy proteins were further identified.It establishes the foundation of finding specific gastric cancer proteins,which act as biomarkers for the diagnosis and prognosis of gastric cancer.